An Investigation of the Effects of Self-Assembled Monolayers on Protein Crystallisation

نویسندگان

  • Chen-Yan Zhang
  • He-Fang Shen
  • Qian-Jin Wang
  • Yun-Zhu Guo
  • Jin He
  • Hui-Ling Cao
  • Yong-Ming Liu
  • Peng Shang
  • Da-Chuan Yin
چکیده

Most protein crystallisation begins from heterogeneous nucleation; in practice, crystallisation typically occurs in the presence of a solid surface in the solution. The solid surface provides a nucleation site such that the energy barrier for nucleation is lower on the surface than in the bulk solution. Different types of solid surfaces exhibit different surface energies, and the nucleation barriers depend on the characteristics of the solid surfaces. Therefore, treatment of the solid surface may alter the surface properties to increase the chance to obtain protein crystals. In this paper, we propose a method to modify the glass cover slip using a self-assembled monolayer (SAM) of functional groups (methyl, sulfydryl and amino), and we investigated the effect of each SAM on protein crystallisation. The results indicated that both crystallisation success rate in a reproducibility study, and crystallisation hits in a crystallisation screening study, were increased using the SAMs, among which, the methyl-modified SAM demonstrated the most significant improvement. These results illustrated that directly modifying the crystallisation plates or glass cover slips to create surfaces that favour heterogeneous nucleation can be potentially useful in practical protein crystallisation, and the utilisation of a SAM containing a functional group can be considered a promising technique for the treatment of the surfaces that will directly contact the crystallisation solution.

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عنوان ژورنال:

دوره 14  شماره 

صفحات  -

تاریخ انتشار 2013